| Block Configuration |
96-Well Fast (10 to 30 uL reactions, optimized for 10 uL reactions) |
| Sample Format |
Fast 96-Well plates (100 uL) or 8-tube strips (100 uL) |
| Temperature Range |
4°C to 99.9°C |
| Temperature Uniformity |
± 1°C |
| Temperature Accuracy |
± 0.5°C |
| Sample Ramp Rate |
Fast mode: 3.5°C/sec Standard mode: 1.6°C/sec |
| Peak Block Heating Rate |
5.5°C/second |
| Run Time |
< 40 minutes |
| Optical System |
CCD camera with halogen lamp excitation; five-exciation and five-emission filters |
| Dynamic Range |
9 logs of linear dynamic range |
| Sensitivity |
Detection of 1 copy of template in 20 uL reaction for a single reporter TaqMan assay, with 99.7% confidence |
| Multiplexing |
Up to 5 targets per well |
| Dye Compatibility |
FAM/SYBR Green, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red, and Cy5 (Calibration for new dyes within the wavelength range is possible by following the custom dye calibration procedure in the user’s manual) |
| Passive Reference Dyes |
ROX dye or any calibrated dye (use of a passive reference dye is optional) |
| Dimensions (W x D x H) |
13.9 x 17.7 x 19.3 in. (34 x 45 x 49 cm) |
| Weight |
75 lb. (34 kg) |
| Electrical Requirements |
100-240V, 50/60Hz, 1080W |
