Agilent 1100 G1323B HPLC Gameboy Controller

Setable Flow Range

Setpoints 0.001 to 5 ml/min, in 0.001 ml/min increments

Flow Range

0.1 to 5 ml/min

Flow Precision

< 0.07% RSD or < 0.02 min SD

Flow Accuracy

± 1% or 10 ul/min

Pressure

Operating range 0 to 400 bar (0 to 5880 psi) up to 5 ml/min

Pressure Pulsation

< 2% amplitude (typically < 1%) at 1 ml/min isopropanol, at all pressures > 1 MPa

Compressibility Compensation

User-selectable, based on mobile phase compressibility

Hydraulic System

Two dual pistons in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve

Recommended pH Range

1.0 to 12.5; solvents with pH < 2.3 should not contain acides with attack stainless steel

Gradient Formation

High-pressure binary mixing, delay volume 180 to 480 ul without mixer, 600 to 900 ul with mixer, dependent upon back pressure

Composition Range

1 to 99% or 5 ul/min per channel

Composition Precision

< 0.5% absolute

Composition Accuracy

< 0.15% RSD at 1 ml/min

Setable Flow Range

0.001 to 10 ml/min, in 0.001 ml/min increments

Flow Range

0.2 to 10.0 ml/min

Flow Precision

< 0.07% RSD, or < 0.02 min SD whatever is greater, based n retention time at constant room temperature

Flow Accuracy

± 1% or 10 µl/min whatever is greater

Pressure

Operating range 0 – 40 MPa (0 – 400 bar, 0 – 5880 psi) up to 5 ml/min

Operating range 0 – 20 MPa (0 – 200 bar, 0 – 2950 psi) up to 10 ml/min

Pressure Pulsation

< 2% amplitude (typically < 1%), at 1 ml/min isopropanol, at all pressures > 1 MPa (10 bar)

Recommended pH Range

1.0 to 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel

Gradient Formation

Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve

Delay volume 800 – 1100 µl, dependent on back pressure

Composition Range

0 to 95% or 5 to 100%, user selectable

Composition Precision

< 0.2% SD, at 0.2 and 1 ml/min

Dimensions (H x W x D)

5.5 x 13.5 x 17 in

Electrical Requirements

100-120 or 22-240 VAC, 50/60 Hz, 220 VA

Trigger Modes

Time slices, Peak (threshold, up- /downslope), Timetable (combination of time intervals and peak) and Manual trigger (supported only with G1323BControl Module)

Operating Modes

Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste

Test Tube/Plate Sizes

Minimum 48 mm to 75 mm maximum

Maximum Tube Volume

ca. 45 ml

Maximum Flow Rate

100 ml / min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve)

Delay Volumes [μl]

Fraction collector inlet to diverter valve: -500 (typical, depends on length of the tubing)

Diverter valve: ~15

Diverter valve to needle: ~110

Needle: -~5

Delay Calibration Sensor

Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode

Diverter Valve

3/2 Diverter valve with low internal volume (15 μl), switching time < 100 ms, maximum operating pressure 6 bar

Maximum Capacity

3 fraction collectors in parallel plus one recovery fraction collector connected via 12-Position, 13-Port Selector valve

Interfaces

Controller-area network (CAN). optional; LAN or external contacts interface RS232C, APG-remote (for remote start / stop signals to / from other modules) Interface to G1330A Thermostat CAN-DC-out for operation of Agilent approved external devices like valves

Safety Features

Leak detection and safe leak handling, error detection and display, exhaust fan for fume extraction of hazardous vapors

Setable Flow Range

0.001 to 10 mL/min, in 0.001 mL/min increments

Flow Range

0.2 to 10.0 mL/min

Flow Precision

≤ 0.07% RSD, or ≤ 0.02 min SD whatever is greater, based on retention time at constant room temperature

Flow Accuracy

± 1% or 10 µl/min whatever is greater

Pressure Operating Range

0 to 60 MPa (0 – 600 bar, 0 – 8,700 psi) up to 5 mL/min

0 to 20 MPa (0 – 200 bar, 0 – 2,950 psi) up to 10 mL/min

Pressure Pulsation

< 2% amplitude or < 3 bar at 1 mL/min isopropanol, at all pressure > 1 MPa (10 bar, 145 psi)

Recommended pH Range

1.0 to 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel

Gradient Formation

Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve

Composition Range

0 to 95% or 5 to 100%, user selectable

Composition Precision

< 0.2% RSD or < 0.04 min SD

Electrical Requirements

100 to 240V, 50/60 Hz, 180 VA

GLP Features

Early maintenance feedback (EMF), electronic records of maintenance and errors

Communications

Controller-area network (CAN), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output

Safety Features

Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display

Injection Range

0.1 to 100 µl in 0.1 µl increments (recommended 1 µl increments)

Up to 1500 µl with multiple draw (hardware modification required)

Replicate Injections

1 to 99 from one vial

Precision

Typically < 0.25 % RSD of peak areas from 5 to 100 µl

Typically < 1 % RSD of peak areas from 1 to 5 µl

Minimum Sample Volume

1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial

Carryover

Typically < 0.1 %, < 0.05 % with external needle cleaning

Sample Viscosity Range

0.2 to 50 cp

Metering Devices

Metering pump in high pressure flow path

Sample Capacity

100 x 2 ml vials in 1 tray

40 x 2 ml vials in ½ tray

15 x 6 ml vials in ½ tray (Agilent vials only)

Injection Cycle Time

50 seconds for draw speed 200 µl/min, ejection speed 200 µl/min, injection volume 5 µl