Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis

10 fg Anthracene, Ex=250 nm, Em=400 nm*
RAMAN (H2O) > 200 (FLF rev A)
RAMAN (H2O) > 400 (FLF rev >A)
Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds response time)

Xenon Flash Lamp, normal mode 20 W, economy mode 5 W

296 Hz for single signal mode, 74 Hz for spectral mode

Range: 200 nm - 700 nm and zero-order
Bandwidth: 20 nm (fixed)
Monochromator:concave holographic grating, F/1.6, blaze: 300 nm

Range: 280 nm - 900 nm and zero-order
Bandwidth: 20 nm (fixed)
Monochromator:concave holographic grating, F/1.6, blaze: 400 nm

up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters

Excitation or Emission spectra
Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200-400 nm, 10 nm step)
Step size: 1-20 nm
Spectra storage: All

Repeatability ±0.2 nm
Accuracy ±3 nm setting

Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz
Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz

Agilent ChemStation for LC, Agilent 1100 Control Module with limited spectral data analysis and printing of spectra

Recorder/integrator: 100 mV or 1 V, output range >102 luminescence units, two outputs

Controller-area network (CAN), GPIB, RS-232C, LAN, APG
Remote: ready, start, stop and shut-down signals

Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.

Early Maintenance Feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy, using the Raman band of water

0 to 40 °C constant temperature at <95% humidity (non-condensing)

140 mm x 345 mm x 435 mm (5.5 x 13.5 x 17 inches) (height x width x depth)
25.5 lbs