Applied Biosystems OpenArray AccuFill System
Fill Rate
99.25%
Maximum Allowable Sample Carryover
< 1%
Maximum Allowable Assay Carryover
< 1%
Features
- Automated transfer of samples and PCR master mix from 384-well plates to OpenArray plates
- Capillary-based loading with self-metering through-holes for consistent reaction volumes
- Bubble-free dispensing enabled by an open-well, through-hole plate design
- Optimized for high-throughput real-time PCR and genotyping workflows
- Compatible with SYBR, OpenArray Real-Time PCR, TaqMan OpenArray Real-Time PCR, and TaqMan OpenArray Genotyping chemistries
- High throughput equivalent to eight traditional 384-well plates per OpenArray plate
- Ability to process up to four OpenArray plates per run for large-scale studies
- Intuitive software for plate setup, sample mapping, file generation, and data integration
- High performance metrics including a typical 99.25% fill rate and minimal sample or assay carryover
The Applied Biosystems OpenArray AccuFill System is an automated, high-throughput sample loading platform designed to precisely transfer DNA samples and PCR master mix from standard 384-well plates onto OpenArray Plates for real-time PCR analysis. The system uses capillary action to draw samples into AccuFill tips and dispense them evenly across the OpenArray plate surface, where self-metering through-holes automatically fill to the correct reaction volume without the need for pressure-based dispensing or manual volume control. Because the OpenArray format contains no closed wells, air bubbles are not trapped during loading, resulting in highly uniform fills and excellent data consistency.
Optimized specifically for downstream real-time PCR workflows, the AccuFill System supports SYBR, OpenArray Real-Time PCR, TaqMan OpenArray Real-Time PCR, and TaqMan OpenArray Genotyping chemistries. OpenArray technology significantly increases throughput and efficiency: a single QuantStudio™ 12K Flex OpenArray plate is equivalent to eight conventional 384-well plates, and the system can process up to four OpenArray plates per run. This enables laboratories to analyze up to 1,728 genotyping samples or 2,304 gene expression samples within an 8-hour workday, with the option for offline thermal cycling to further optimize instrument utilization.
The accompanying OpenArray AccuFill v2.0 software provides end-to-end workflow control, including sample plate setup, mapping from 96-well to 384-well formats, support for multi-sample loading, and generation of all required plate files (TPF, SPF, loaded files, and CSV outputs) for compatible Applied Biosystems™ real-time PCR instruments. The software also includes plate orientation correction, loading history tracking, and sample integration capabilities to streamline data management. Designed for reliability and reproducibility, the system delivers a typical fill rate of 99.25%, with maximum allowable sample and assay carryover below 1% and less than 5% data loss due to evaporation, making it a robust solution for high-density, high-confidence qPCR applications
All of our equipment is fully tested to perform at factory specifications before being shipped.
INCLUDED:
The Applied Biosystems OpenArray AccuFill System includes:
The Applied Biosystems OpenArray AccuFill System includes:
- > OpenArray Plate Press 2
- > Waste Bin
- > Windows 11 Professional 64-Bit Data Station with Flatscreen Monitor
- > OpenArray AccuFill v2.0 Software Installed
- > Power Cord
- > Operator's Manual
- > Full 180 Day Parts and Labor Warranty
Applied Biosystems OpenArray AccuFill System Specifications
| Fill Rate | 99.25% |
| Maximum Allowable Sample Carryover | < 1% |
| Maximum Allowable Assay Carryover | < 1% |
| Maximum Number of Through-Hole Data Points Lost Due to Evaporation | < 5% |
| Dimensions (H x D x W) | 34.5 x 40 x 42 in. (87.6 x 101.6 x 106.7 cm) |
| Weight | 243 lb. (110.2 kg) |
| Electrical Requirements | 100 to 240VAC, 50/60Hz, 4A |
|
Computer Specifications |
|
| Processor | Intel Core I5-8500 CPU |
| RAM | 32.0 GB |
| Hard Drive | 500GB Internal SSD |
| Operating System | Windows 11 Professional, 64-bit |
Thermal Cycler Inspection and Recertification Checklist
- Validate that the power supply and PCB consistently deliver accurate, stable voltages across all circuits and connections, confirming operational reliability
- Thoroughly inspect the display for any dim, flickering, or dead pixels, replacing compromised segments to ensure clear visibility and precise data display
- Verify complete functionality and responsiveness of all buttons and knobs, confirming accurate and reliable input across all controls
- Ensure the machine completes all internal diagnostic checks with zero errors, indicating system readiness and functional integrity
- Confirm the machine accurately identifies attached blocks (96-well, dual 48, etc.), with seamless recognition across different configurations
- Conduct a detailed inspection of internal wiring and all electrical connections, checking for any signs of breaks, disconnections, or tears; replace or repair any underperforming components
- Examine the connector between the block and base for bent or broken pins, replacing as needed to maintain secure, reliable connections
- Calibrate well temperatures with a NIST-traceable thermometer, verifying accuracy and uniformity across all wells to meet specification standards
- Perform a comprehensive cosmetic inspection, ensuring all exterior surfaces and components are free of damage, wear, or defects, maintaining aesthetic and professional quality
Technician Inspection and Recertification Checklist: Thermal Cycler
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment| Task | Completed? | Remarks? |
| Validate stable, accurate voltage outputs from power supply and PCB across circuits | Completed | |
| Inspect display for dim, flickering, or dead pixels; replace as needed for clarity | Completed | |
| Verify functionality and responsiveness of all buttons and knobs | Completed | |
| Confirm machine completes internal diagnostics with zero errors | Completed | |
| Ensure accurate block identification across configurations (96-well, dual 48, etc.) | Completed | |
| Inspect internal wiring and connections for integrity; repair or replace as needed | Completed | |
| Check connector between block and base for bent or broken pins; replace as necessary | Completed | |
| Calibrate well temperatures with a NIST-traceable thermometer for accuracy | Completed | |
| Conduct cosmetic inspection to ensure quality and professionalism of all surfaces | Completed |
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