Applied Biosystems ViiA 7 Real-Time PCR System w/ 384-Well Sample Block
Block Configuration
384-Well (5 to 20 uL reactions)
Run Time
~ 35 Minutes
Excitation/Detection Wavelengths
EX: 450 to 670 nm / EM: 500 to 720 nm (Filter-based)
Features
- OptiFlex system with six excitation and six emission filters for high-resolution detection
- Detects as little as 1.5-fold changes in singleplex target quantities
- Front-access block change in under one minute with no tools required
- Intuitive 7" touchscreen interface with one-touch protocol setup
- Compatible with TaqMan Array Micro Fluidic Cards for high-throughput workflows
- Supports up to nine logs of linear dynamic range and single-copy sensitivity
- Automation-ready and integrates with the Twister II Robot for scalable throughput
- Built-in data recovery for over 100 runs ensures workflow continuity
- Fully configurable data export formats for flexible downstream analysis
The Applied Biosystems ViiA 7 Real-Time PCR System with 384-Well Sample Block is a high-throughput qPCR platform designed to deliver exceptional performance, reliability, and flexibility for demanding research environments. Engineered with a front-access interchangeable block system, it allows for rapid block changes in under one minute without tools or disconnecting peripherals. The 384-well format supports reaction volumes from 5 to 20 µL and enables fast run times of approximately 35 minutes, making it ideal for medium- to high-throughput applications. At the core of the system is the advanced OptiFlex fluorescence detection module, featuring six independent excitation (450 - 670 nm) and six emission (500 - 720 nm) filters, offering unmatched multiplexing capabilities and precise quantification, detecting target changes as small as 1.5-fold in singleplex reactions.
The ViiA 7 System supports a fully integrated workflow, with compatibility for standard and fast 96-well plates, 384-well plates, as well as TaqMan® Array Micro Fluidic Cards, allowing users to perform hundreds of assays without the need for liquid-handling robotics. A user-friendly 7" color touchscreen interface, one-touch protocols, and automation readiness via the Twister II Robot (not included) ensure streamlined operation and scalability. Enhanced software tools include customizable export formats, ramp rate detection flexibility, and robust data recovery capabilities for over 100 runs. With a dynamic range extending up to nine logs and the sensitivity to detect down to a single copy, the ViiA 7 delivers high-quality, reproducible results instrument to instrument and run to run, empowering researchers to achieve more, faster. Please Note: We recommend performing a Dye Calibration for the Dye you plan on using for your application(s). Please refer to the Operator's Manual for instructions.
All of our equipment is fully tested to perform at factory specifications before being shipped.
INCLUDED:
The Applied Biosystems ViiA 7 Real-Time PCR System w/ 384-Well Sample Block includes:
The Applied Biosystems ViiA 7 Real-Time PCR System w/ 384-Well Sample Block includes:
- > 384-Well Block
- > 384-Well Heated Cover
- > Background Calibration Report
- > ROI Calibration Report
- > Uniformity Calibration Report
- > Windows 11 Professional 64-Bit Data Station with Flatscreen Monitor
- > QuantStudio Real-Time PCR Software Version 1.6.1 Installed
- > Power Cord
- > Operator's Manual
- > Full 180 Day Parts and Labor Warranty
Applied Biosystems ViiA 7 Real-Time PCR System w/ 384-Well Sample Block Specifications
| Block Configuration | 384-Well (5 to 20 uL reactions) |
| Block Change Design | Block change from front of instrument in less than 1 minute; no secondary tools required |
| Run Time | ~ 35 minutes for 384-Well |
| Demonstrated Sensitivity | Down to 1 copy |
| Dynamic Range | Up to 9 logs of linear dynamic range |
| Resolution | Detect changes as little as 1.5-fold in target quantities in singleplex reaction |
| Excitation/Detection Wavelengths |
6 Excitation: 450 to 670 nm 6 Emission: 500 to 720 nm |
| Data Export Format |
Fully configurable by user |
| Dimensions (W x D x H) | 21 x 25 x 25.4 in. (53.3 x 63.5 x 64.5 cm) |
| Weight | 148 lb. (66.9 kg) |
| Electrical Requirements | 100-240V, 50/60Hz, 12.5A |
|
Computer Specifications |
|
| Processor | Intel Core I5-8500 CPU |
| RAM | 32.0 GB |
| Hard Drive | 500GB Internal SSD |
| Operating System | Windows 11 Professional, 64-bit |
Thermal Cycler Inspection and Recertification Checklist
- Validate that the power supply and PCB consistently deliver accurate, stable voltages across all circuits and connections, confirming operational reliability
- Thoroughly inspect the display for any dim, flickering, or dead pixels, replacing compromised segments to ensure clear visibility and precise data display
- Verify complete functionality and responsiveness of all buttons and knobs, confirming accurate and reliable input across all controls
- Ensure the machine completes all internal diagnostic checks with zero errors, indicating system readiness and functional integrity
- Confirm the machine accurately identifies attached blocks (96-well, dual 48, etc.), with seamless recognition across different configurations
- Conduct a detailed inspection of internal wiring and all electrical connections, checking for any signs of breaks, disconnections, or tears; replace or repair any underperforming components
- Examine the connector between the block and base for bent or broken pins, replacing as needed to maintain secure, reliable connections
- Calibrate well temperatures with a NIST-traceable thermometer, verifying accuracy and uniformity across all wells to meet specification standards
- Perform a comprehensive cosmetic inspection, ensuring all exterior surfaces and components are free of damage, wear, or defects, maintaining aesthetic and professional quality
Technician Inspection and Recertification Checklist: Thermal Cycler
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment| Task | Completed? | Remarks? |
| Validate stable, accurate voltage outputs from power supply and PCB across circuits | Completed | |
| Inspect display for dim, flickering, or dead pixels; replace as needed for clarity | Completed | |
| Verify functionality and responsiveness of all buttons and knobs | Completed | |
| Confirm machine completes internal diagnostics with zero errors | Completed | |
| Ensure accurate block identification across configurations (96-well, dual 48, etc.) | Completed | |
| Inspect internal wiring and connections for integrity; repair or replace as needed | Completed | |
| Check connector between block and base for bent or broken pins; replace as necessary | Completed | |
| Calibrate well temperatures with a NIST-traceable thermometer for accuracy | Completed | |
| Conduct cosmetic inspection to ensure quality and professionalism of all surfaces | Completed |
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customerservice@marshallscientific.com | (603) 601-8511 |
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