Biorad MyCycler Thermal Cycler
Temperature Range
4°C to 100°C
Ramping Rates
Heating: 2.5°C/sec - Cooling: 1.5°/sec
Gradient Capability
Yes
- Compact 96-well PCR system with gradient feature for gene amplification
- Intuitive programming with templates for easy protocol setup and editing
- Capable of programming up to 9 cycles with 9 steps each, repeating up to 99 times per cycle
- Self-adjusting heated lid to maintain accurate temperature for standard 0.2 ml thin-wall PCR tubes, strips, and plates
- Offers temperature monitoring and control via algorithm or block modes
- Rapid cycling speed with a maximum heating rate of at least 2.5°C/sec and cooling rate of 1.5°C/sec
- Temperature range from 4-100°C with well-to-well uniformity of ±0.5°C
- Functional temperature gradient ranging from 5-25°C, with a gradient accuracy of ±0.5°C
All of our equipment is fully tested to perform at factory specifications before being shipped.
The Biorad MyCycler Thermal Cycler includes:
- > 96 Well Alpha Block
- > Power Cord
- > Calibration and Preventative Maintenance Report
- > Operator's Manual
- > Full 180 Day Parts and Labor Warranty
Biorad MyCycler Thermal Cycler Specifications
|
Block Temperatures |
|
|
Range |
4-100°C |
|
Cycling Speed |
With 20 ul sample in a 0.2 ml tube, after warm up, when cycling between 50°C and 95°C; at least 2.5°C/sec maximum heating rate and 1.5°C/sec maximum cooling rate |
|
Storage Cooling |
Less than 3 minutes to cool from 40°C to 4°C |
|
Overshoot |
Less than0.5°C |
|
Accuracy |
±0.5°C to NIST traceable standard after 30 seconds at 90°C, to block mode |
|
Uniformity |
Well to well uniformity of ±0.5°C within 30 seconds after arrival at 90°C |
|
Temperature Gradient |
|
|
Input Gradient Range |
1-25°C |
|
Functional gradient range |
5-25°C |
|
Mininum temperature of gradient |
30°C |
|
Maximum temperature of gradient |
99°C |
|
Gradient accuracy |
±0.5°C |
|
Gradient Uniformity |
0.5°C |
- Validate that the power supply and PCB consistently deliver accurate, stable voltages across all circuits and connections, confirming operational reliability
- Thoroughly inspect the display for any dim, flickering, or dead pixels, replacing compromised segments to ensure clear visibility and precise data display
- Verify complete functionality and responsiveness of all buttons and knobs, confirming accurate and reliable input across all controls
- Ensure the machine completes all internal diagnostic checks with zero errors, indicating system readiness and functional integrity
- Confirm the machine accurately identifies attached blocks (96-well, dual 48, etc.), with seamless recognition across different configurations
- Conduct a detailed inspection of internal wiring and all electrical connections, checking for any signs of breaks, disconnections, or tears; replace or repair any underperforming components
- Examine the connector between the block and base for bent or broken pins, replacing as needed to maintain secure, reliable connections
- Calibrate well temperatures with a NIST-traceable thermometer, verifying accuracy and uniformity across all wells to meet specification standards
- Perform a comprehensive cosmetic inspection, ensuring all exterior surfaces and components are free of damage, wear, or defects, maintaining aesthetic and professional quality
Technician Inspection and Recertification Checklist: Thermal Cycler
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment| Task | Completed? | Remarks? |
| Validate stable, accurate voltage outputs from power supply and PCB across circuits | Completed | |
| Inspect display for dim, flickering, or dead pixels; replace as needed for clarity | Completed | |
| Verify functionality and responsiveness of all buttons and knobs | Completed | |
| Confirm machine completes internal diagnostics with zero errors | Completed | |
| Ensure accurate block identification across configurations (96-well, dual 48, etc.) | Completed | |
| Inspect internal wiring and connections for integrity; repair or replace as needed | Completed | |
| Check connector between block and base for bent or broken pins; replace as necessary | Completed | |
| Calibrate well temperatures with a NIST-traceable thermometer for accuracy | Completed | |
| Conduct cosmetic inspection to ensure quality and professionalism of all surfaces | Completed |
Downloads


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