Eppendorf 5425 Centrifuge

Features

• Aerosol-tight Eppendorf QuickLock® lids for simple and safe handling.
• Outstanding software features with adjustable acceleration and braking ramps, 3 program buttons, and short function without continuous pressing of the key.
• OptiBowl® design for quiet operation, even without a rotor lid.
• Soft-touch one-finger lid closure for ergonomic operation.
• Max. rotor capacity: 10 × 5.0 mL vessels.

The Eppendorf 5425 Centrifuge is a whisper quiet microcentrifuge offering an outstanding set of features for a broad range of molecular and cell biology applications. Short runs up to 12 minutes are whisper quiet at an average noise level of 45 dB(A) and maximum RCF of up to 21,300 x g. This is sufficient for centrifugation steps of most routinely used DNA/RNA purification kits. The 5425 also features a wide speed range, with a minimum speed of 100 RPM and a maximum speed of 15,060 RPM. With a comprehensive range of 6 rotors as well, this broadens the spectrum of applications of the 5425. The included FA-24x2 Fixed Angle Rotor reaches a maximum speed of 15,060 RPM.

Eppendorf 5425 Centrifuge Specifications

Centrifuge Inspection and Recertification Checklist

• Confirm the power supply output voltages match specified requirements for stable distribution to all internal components
• Measure and verify voltages at PCB test points to ensure accurate power delivery and circuit integrity
• Confirm rotor compatibility with the centrifuge model and check that rotor detection systems recognize the rotor type
• Inspect the exterior for any cosmetic or structural damage that could affect performance or safety
• Check the door mechanism alignment to ensure secure and smooth opening and closing
• Inspect and replace the gas strut with an OEM part to maintain proper door support and safety
• Examine the rotor chamber bowl for dents or damage and replace as needed to ensure safe operation
• Replace and confirm full functionality of all lid lock switches to maintain secure closure
• Inspect and lubricate the door lock mechanism, applying grease to the lid lock rail for smooth operation
• Test compressor functionality (if applicable) to ensure proper operation and consistent temperature control
• Conduct an 8-12 hour cycle test to validate consistent performance over prolonged use
• Confirm rotor motor stability, check for unusual noise, and assess mechanical integrity during operation
• Inspect the condensation drain and clear any clogs to prevent moisture buildup in the rotor chamber
• Verify RPM accuracy using an NIST-traceable tachometer and perform calibration as necessary
• Check the centrifuge error log to ensure zero recorded issues, confirming system reliability

Technician Inspection and Recertification Checklist: Centrifuges

The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment

Task	Completed?	Remarks?
Confirm power supply output voltages match specified requirements	Completed
Measure and verify voltages at PCB test points for accuracy	Completed
Confirm rotor compatibility and detection for proper identification	Completed
Inspect exterior for cosmetic or structural damage	Completed
Check door mechanism alignment for secure opening and closing	Completed
Inspect and replace gas strut with OEM part as needed	Completed
Examine rotor chamber for dents or damage and replace if necessary	Completed
Replace and confirm functionality of all lid lock switches	Completed
Lubricate door lock mechanism and lid lock rail for smooth operation	Completed
Test compressor (if applicable) for proper operation and cooling	Completed
Run 8-12 hour cycle test to ensure consistent performance	Completed
Check rotor motor for stability, noise, and integrity	Completed
Inspect and clear condensation drain to prevent buildup	Completed
Verify RPM accuracy with NIST traceable tachometer and calibrate	Completed
Check centrifuge error log for zero recorded issues	Completed

102 Tide Mill Road, Hampton NH 03842
customerservice@marshallscientific.com | (603) 601-8511 |
www.marshallscientific.com
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Preventative Maintenance Performed on The Eppendorf 5425 Centrifuge

• Door Latch Assembly Checked for Full Function
• Door Hinges are Checked and Lubed as Needed
• Drive Belt is Checked for Rips and Tears and Replaced if Needed
• Rotors and O-rings are Lubed as Needed
• Lamps and Display are Checked and Replaced if Dim
• Line Cord is Checked Signs of Fray or Damage and Replaced Accordingly
• Internal Circuitry is Checked for Faulty/Shorted Components and Replaced Accordingly