GE AKTA Prime Plus FPLC System
Flow Rate Range
0.1 to 50 mL/min
Maximum Operating Pressure
1 MPa (145 psi)
Single Wavelength Detection
254 and 280 nm Filters Included
- Versatile chromatography system designed for single-step purifications of proteins and antibodies
- Compatible with prepacked columns like HiTrap, HiPrep, and HiLoad for easy setup
- Offers high reproducibility in a compact and user-friendly design with pre-programmed templates
- Flow rate ranges from 0.1 mL/min to 50 mL/min, accommodating various purification scales
- Maximum operating pressure of 1 MPa (145 psi) for robust performance
- Includes single-wavelength detection with a mercury lamp and filters for 254 and 280 nm
- Features two buffer inlets for flexible buffer selection during purification processes
- Designed to work within a relative humidity range of 20% to 95% (non-condensing) and pH stability long term of 2 to 12
The GE AKTA Prime Plus FPLC System includes:
- > Windows 11 Professional 64-Bit Data Station with Pre-Loaded PrimeView Software
- > Conductivity Flow Cell
- > Built in Fraction Collector
- > UV Lamp & Detector
- > Power Cords and Cables
- > Installation Calibration Report
- > Operator's Guide
- > Full 180 Day Parts and Labor Warranty
GE AKTA Prime Plus FPLC System Specifications
|
Flow Rate |
0.1mL/min to 50mL/min |
|
Maximum Operating Pressure |
1 MPa (145 psi) |
|
Tubing ID (Flow Path) |
0.75 mm |
|
Single Wavelength Detection |
Mercury lamp and 254 and 280 nm filters included. Optional filters for 313, 365, 405, 436, and 546 nm (through manufacturer). Optional zink lamp with 214 nm filter (through manufacturer). |
|
Flow Cell Path Length |
2 mm (optional 5 mm through manufacturer) |
|
Buffer Selection |
2 inlets |
|
Relative Humidity |
20% to 95% (non-condensing) |
|
pH Stability Long Term |
2 to 12 |
|
Viscosity |
Max 10 cP (< 10 ml/min), max 5 cP (>10 ml/min) |
|
Dimensions (W x H x D) |
400 x 530 x 250 mm |
|
Computer Specifications |
|
| Processor | Intel Core I5-8350U CPU |
| RAM | 16.0 GB |
| Hard Drive | 512GB Internal SSD |
| Operating System | Windows 11 Professional, 64-bit |
- Inspect all displays, replacing any dim or dead pixels to ensure optimal visual feedback
- Examine internal components of P-900, UV-900/UPC-900, and pH/C-900, replacing any malfunctioning parts to maintain performance
- Verify connections of all components via Uni-Net 1 & 2 interfaces for secure and reliable communication
- Check Uni-Net cables, replacing any faulty ones to ensure uninterrupted data transmission
- Inspect internal components of M-925, INV-907, PV-908, and PV-907 to confirm full operational functionality
- Replace all PEEK tubing with new OEM tubing and inspect all components within the flow path for potential clogs
- Confirm successful software interfacing with the FPLC system for seamless operation
- Replace pump piston seals, wash seals, and seal wash tubing, performing ultrasonic cleaning on each check valve in the pump flow path
- Calibrate pressure and flow rates of both the P-900 pump and P-960/950 pump (if applicable) for precise control
- Replace UV lamp and perform internal inspection tests to confirm complete functionality
- Install a new pH probe and verify the conductivity cell for accurate readings
- Calculate and adjust delay volume in software to reflect system configuration
- Clean or replace the drop sensor, accumulator wash, and height adjuster for consistent collection performance
- Verify fraction collector alignment and calibration with 15mL, 50mL, and microplate receptacles to ensure accuracy
- Run installation tests and thoroughly document all findings for comprehensive record-keeping
Technician Inspection and Recertification Checklist: FPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment| Task | Completed? | Remarks? |
| Inspect all displays and replace dim/dead pixels | Completed | |
| Examine internal components of P-900, UV-900/UPC-900, and pH/C-900 | Completed | |
| Verify connections via Uni-Net 1 & 2 interfaces | Completed | |
| Check and replace any malfunctioning Uni-Net cables | Completed | |
| Inspect internal components of M-925, INV-907, PV-908, PV-907 | Completed | |
| Replace PEEK tubing with OEM tubing and check for clogs | Completed | |
| Confirm software interfacing with FPLC system | Completed | |
| Replace pump seals and wash tubing; clean check valves | Completed | |
| Calibrate pressure and flow rates of P-900 and P-960/950 pumps | Completed | |
| Replace UV lamp and confirm full functionality | Completed | |
| Install new pH probe and verify conductivity cell | Completed | |
| Calculate delay volume and update in software | Completed | |
| Clean or replace drop sensor, accumulator wash, and height adjuster | Completed | |
| Verify fraction collector alignment with 15mL, 50mL, and microplate receptacles | Completed | |
| Run installation tests and document all findings | Completed |
102 Tide Mill Road, Hampton NH 03842
customerservice@marshallscientific.com | (603) 601-8511 |
www.marshallscientific.com
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