Thermo Scientific NanoDrop 8000 Spectrophotometer
Absorbance Range
0 to 75 Abs (10 mm equivalent)
Minimum Sample Size
1 uL
Wavelength Range
220 and 750 nm
- Capable of measuring up to eight 1 uL samples simultaneously
- Patented sample retention technology eliminates the need for cuvettes
- Measures highly concentrated samples without dilution
- Suitable for nucleic acid, protein analysis, and general UV-Vis spectrophotometry
- Xenon flash lamp and silicon photodiode detector ensure precise detection
- Spectral resolution of ≤ 3 nm
- Rapid measurement capability, completing analysis in less than 20 seconds
- Constructed with durable 303 stainless steel and quartz fiber materials
The Thermo Scientific NanoDrop 8000 Spectrophotometer stands out as a highly efficient, full-spectrum (220-750 nm) analytical instrument capable of accurately measuring up to eight 1 μl samples in a single cycle. Utilizing innovative sample retention technology, which leverages surface tension to secure samples without the need for cuvettes, this spectrophotometer simplifies operations and expedites cleanup. The device's versatility is further enhanced by its ability to handle a wide absorbance range from 0 to 75 Abs, with an auto-ranging path length between 0.1, 0.2, and 1.0 mm, accommodating both highly concentrated and minute sample volumes.
Equipped with a xenon flash lamp and a silicon photodiode detector, the NanoDrop 8000 delivers exceptional spectral resolution of ≤3 nm, ensuring precise measurements. It's uniquely suited for a variety of applications including the quantitation of nucleic acids and proteins, fluorescent dye labeling density assessments for microarray samples, and general UV-Vis spectrophotometry. The spectrophotometer is especially effective for direct concentration measurements of nucleic acid up to 3700 ng/ul and protein up to 100 mg/ml without the need for dilution.
Noteworthy for its speed and efficiency, the NanoDrop 8000 can complete a measurement in less than 20 seconds, making it an indispensable tool for fast-paced research environments. The robust design incorporates a durable sample pedestal made from 303 stainless steel and quartz fiber, enhancing its longevity and reliability in demanding laboratory settings. This combination of speed, precision, and versatility makes the NanoDrop 8000 an essential piece of equipment for scientific researchers who require accurate, reproducible results in their spectroscopic analyses.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The Thermo Scientific NanoDrop 8000 Spectrophotometer includes:
- > Windows 11 Pro Data Station with Nanodrop 8000 Software Preloaded
- > CF-1 Calibration Report
- > Power Cord
- > Full 180 Day Parts and Labor Warranty
Thermo Scientific NanoDrop 8000 Spectrophotometer Specifications
| Wavelength Range | 220 to 750 nm |
| Absorbance Accuracy | 3% (at 0.74 Abs at 350 nm) |
| Absorbance Range | 0 to 75 Abs (10 mm equivalent) |
| Minimum Sample Size | 1 uL |
| Pathlength | 0.1, 0.2 and 1.0 mm, auto-ranging |
| Light Source | Xenon flash lamp |
| Detector Type | Silicon photodiode |
| Spectral Resolution | ≤ 3 nm (FWHM at Hg 546.1 nm) |
| Lower Limit of Detection |
2.5 ng/uL (dsDNA) 0.15 mg/mL (BSA) |
| Maximum Concentration |
3,700 ng/uL (dsDNA) 100 mg/mL (BSA) |
| Measurement Time | < 20 seconds |
| Sample Pedestal Material | 303 stainless steel and quartz fiber |
| Dimensions | 9.4 x 12.6 in. (24 x 32 cm) |
| Weight | 7.5 lb. (3.4 kg.) |
| Electrical Requirements | 12V DC, 30W |
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Computer Specifications |
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| Processor | Intel Core I5-8500 CPU |
| RAM | 32.0 GB |
| Hard Drive | 500GB Internal SSD |
| Operating System | Windows 11 Professional, 64-bit |
- Confirm the power supply and PCB output correct, stable voltages across all required connections
- Inspect the display for any dim or dead pixels; replace affected components to maintain full visual clarity and operational accuracy
- Verify full functionality of all buttons, ensuring precise and responsive input control
- Check the XY assembly for proper movement and alignment; thoroughly clean all railings, removing any debris or contaminants
- Confirm stable software communication and connection between the instrument and PC, addressing any connectivity issues
- Inspect and clean or replace all filters as required to ensure optimal airflow and performance
- Examine internal wiring and electrical connections for any breaks, disconnections, or tears; replace or secure any compromised wiring
- Test the lamp’s full functionality, replacing it if any performance issues, dimming, or faults are detected
- Execute the necessary validation protocol, documenting completion with a date on a calibration/verification certificate
- Perform and verify appropriate updates to the PC operating system for compatibility and security
- Conduct a thorough cosmetic inspection, ensuring all external components meet quality and aesthetic standards
Technician Inspection and Recertification Checklist: Instruments
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment| Task | Completed? | Remarks? |
| Confirm stable, correct voltage outputs from power supply and PCB | Completed | |
| Inspect display for dim or dead pixels; replace as needed | Completed | |
| Verify functionality of all buttons for precise, responsive control | Completed | |
| Check XY assembly for proper movement; clean railings to remove debris | Completed | |
| Confirm stable software communication between instrument and PC | Completed | |
| Inspect and clean or replace filters to maintain optimal performance | Completed | |
| Examine internal wiring for damage; replace or secure compromised connections | Completed | |
| Test lamp functionality; replace if dimming or faults are detected | Completed | |
| Execute validation protocol and document on calibration/verification certificate | Completed | |
| Update PC operating system for compatibility and security | Completed | |
| Conduct cosmetic inspection to ensure quality of external components | Completed |


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