Thermo Scientific NanoDrop One Microvolume Spectrophotometer
Minimum Sample Size
1 uL
Wavelength Range
190 to 850 nm
Wavelength Accuracy
± 1 nm
- Acclaro Sample Intelligence Technology identifies contaminants and ensures data integrity
- Extended auto-range pathlength enables accurate readings up to 27,500 ng/µL (dsDNA)
- High-resolution 7-inch touchscreen interface with intuitive standalone operation
- No consumables required thanks to the direct pipetting pedestal design
- Fast 8-second measurement time with auto-measure and preloaded workflows
- Xenon flash lamp and 2048-element CMOS sensor for precise UV-Vis analysis
- Multiple connectivity options including USB, Ethernet, Wi-Fi, and Bluetooth with 32 GB internal memory
The Thermo Scientific NanoDrop One Microvolume Spectrophotometer is an advanced, compact UV-Vis instrument designed to deliver fast, reliable quantification of nucleic acids, proteins, and other biomolecules using as little as 1-2 µL of sample. With its integrated Thermo Scientific Acclaro Sample Intelligence Technology, the NanoDrop One provides enhanced sample analysis through real-time contaminant identification, corrected concentration values, and immediate feedback on sample quality. These capabilities help users make confident decisions before moving to downstream applications. The instrument features a sleek, standalone design with a 7-inch high-definition touchscreen interface, eliminating the need for a connected PC and maximizing bench space efficiency.
The xenon flash lamp and 2048-element CMOS sensor provide fast, accurate results with a spectral resolution of ≤ 1.8 nm and a measurement time of just 8 seconds. Built-in Auto-Measure functionality and pre-programmed applications simplify routine workflows. For data management and transfer, the instrument includes three USB-A ports, Ethernet, Bluetooth, Wi-Fi connectivity, and 32 GB of internal flash memory. The NanoDrop One is the ideal choice for researchers requiring high-performance, intelligent microvolume analysis with the added flexibility of cuvette-based capabilities.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The Thermo Scientific NanoDrop One Microvolume Spectrophotometer includes:
- > PV-1 Calibration Report
- > Power Cord
- > Full 180 Day Parts and Labor Warranty
Thermo Scientific NanoDrop One Microvolume Spectrophotometer Specifications
| Wavelength Range | 190 to 850 nm |
| Wavelength Accuracy | ± 1 nm |
| Photometric Range | 0 to 550 A |
| Photometric Accuracy | 3% at 0.97 A, 302 nm |
| Minimum Sample Size | 1 uL |
| Pathlength | 0.030 to 1.0 mm auto-ranging |
| Light Source | Xenon flash lamp |
| Detector | 2048-element CMOS linear image sensor |
| Resolution (Spectral Bandwidth) | ≤ 1.8 nm (FWHM at Hg 254 nm) |
| Lower Limit of Detection |
2.5 ng/uL (dsDNA) 0.15 mg/mL (BSA) |
| Maximum Concentration |
27,500 ng/uL (dsDNA) 820 (400) mg/mL (BSA lgG) |
| Measurement Time | 8 seconds |
| Connectivity | Three USB-A ports, ethernet, Bluetooth, and Wi-Fi |
| Display | 7 inch touchscreen, 1280 x 800 high-definition color display |
| Internal Storage | 32 GB flash memory |
| Dimensions (W x D x H) | 8 x 10 x 12.7 in. (20 x 25.4 x 32.3 cm) |
| Weight | 7.9 lb. (3.6 kg.) |
| Electrical Requirements | 12V DC, 12-18W |
- Confirm the power supply and PCB output correct, stable voltages across all required connections
- Inspect the display for any dim or dead pixels; replace affected components to maintain full visual clarity and operational accuracy
- Verify full functionality of all buttons, ensuring precise and responsive input control
- Check the XY assembly for proper movement and alignment; thoroughly clean all railings, removing any debris or contaminants
- Confirm stable software communication and connection between the instrument and PC, addressing any connectivity issues
- Inspect and clean or replace all filters as required to ensure optimal airflow and performance
- Examine internal wiring and electrical connections for any breaks, disconnections, or tears; replace or secure any compromised wiring
- Test the lamp’s full functionality, replacing it if any performance issues, dimming, or faults are detected
- Execute the necessary validation protocol, documenting completion with a date on a calibration/verification certificate
- Perform and verify appropriate updates to the PC operating system for compatibility and security
- Conduct a thorough cosmetic inspection, ensuring all external components meet quality and aesthetic standards
Technician Inspection and Recertification Checklist: Instruments
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment| Task | Completed? | Remarks? |
| Confirm stable, correct voltage outputs from power supply and PCB | Completed | |
| Inspect display for dim or dead pixels; replace as needed | Completed | |
| Verify functionality of all buttons for precise, responsive control | Completed | |
| Check XY assembly for proper movement; clean railings to remove debris | Completed | |
| Confirm stable software communication between instrument and PC | Completed | |
| Inspect and clean or replace filters to maintain optimal performance | Completed | |
| Examine internal wiring for damage; replace or secure compromised connections | Completed | |
| Test lamp functionality; replace if dimming or faults are detected | Completed | |
| Execute validation protocol and document on calibration/verification certificate | Completed | |
| Update PC operating system for compatibility and security | Completed | |
| Conduct cosmetic inspection to ensure quality of external components | Completed |


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