Agilent 1100 G1323B HPLC Gameboy Controller

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Agilent 1100 G1323B HPLC Gameboy Controller

The Agilent 1100 G1323B Gameboy HPLC Controller is for use with HP/Agilent 1100 and 1200 HPLC systems. The Agilent hand-held control module self-configures all modules for total system control using controller-area network (CAN) communication and cabling. All instrument control parameters including column ID can be stored on credit-card sized PCMCIA (PC) cards for error-free transfer between instruments. A liquid-crystal display allows online monitoring of the signal during the run.

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Maximum Flow Rate	10 ml/min per channel Reduced performance above 5 ml/min
Number of Channels	4
Internal Volume Per Channel	1ml per channel
Materials in Contact with Solvent	PTE, PEEK
pH Range	1 to 14

Type	Specification
Weight	0.8 kg
Dimensions (width x depth x height)	130 x 225 x 35 mm (5.1 x 8.9 x 1.4 inches)
Line voltage	22 VDC, Â± 10 %
Power consumption	6 W / 20.5 BTU/hour
Ambient operating temperature	0 to 55C (32 to 131F)
Ambient non-operating temperature	-40 to 70C (-40 to 158F)
Humidty	< 95%, at 25 â€š 40C (77 to 104F)
Operating Altitude	Up to 2000 m (6562 ft)
Non-operating altitude	Up to 4600 m (15092 ft)
Safety standards: IEC, CSA, UL, EN	Installation category II, pollution degree 2. For indoor use only. Research Use Only. Not for use in Diagnostic Procedures.

Maximum Flow Rate	0 to 5 ml/min per channel 5 to 10 ml/min at reduced degassing performance
Number of Channels	4
Internal Volume Per Channel	Typically 1ml per channel
Materials in Contact with Solvent	PTFE, FEP, PEEK
pH Range	1 to 14

Maximum Flow Rate	10 ml/min per channel
Number of Channels	4
Internal Volume Per Channel	Typically 12ml per channel
Materials in Contact with Solvent	PTFE, PEEK
pH Range	1 to 14

Temperature Range	4Â°C to 40Â°C
Temperature Accuracy at Ambient Temperatures (< 25 Â°C and humidity < 50%)	-1Â°C to +4Â°C at a setpoint of 4 Â°C
Temperature Accuracy at Ambient Temperatures (< 25Â°C and Humidity < 60%)	-1Â°C to +5Â°C at a setpoint of 4 Â°C
Weight	46 lbs. (20.7 kg)

Setable Flow Range	0.001 to 10 ml/min, in 0.001 ml/min increments
Flow Range	0.2 to 10.0 ml/min
Flow Precision	< 0.3% RSD (usually 0.15%), based on retention time, at 1 ml/min
Pressure	Operating range 0 to 40 MPa (0 to 400 bar, 0 to 5880 psi) up to 5 ml/min Operating range 0 to 20 MPa (0 to 200 bar, 0 to 2950 psi) up to 10 ml/min
Pressure Pulsation	< 2% amplitude (typically < 1%), at 1 ml/min isopropanol, at all pressures > 1 MPa (10 bar)
Recommended pH Range	1.0 to 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel
Gradient Formation	Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve Delay volume 800 to 1100 Âµl, dependent on back pressure
Composition Range	0 to 95% or 5 to 100%, user selectable
Composition Precision	< 0.2% SD, at 0.2 and 1 ml/min
Dimensions (H x W x D)	5.5 x 13.5 x 17 in
Electrical Requirements	100 to 120 or 22 to 240 VAC, 50/60 Hz, 220 VA

Detection Type	1024-element photodiode array
Light Source	Deuterium and tungsten lamps
Wavelength Range	190 - 640 nm
Short Term Noise (ASTM) Single and Multi-Wavelength	< Â± 3 x 10^-6 AU at 230 nm/4 nm
Drift	< 0.5 x 10^-3 AU/hr at 230 nm
Linear Absorbance Range	> 2 AU (5%) at 265 nm
Wavelength Accuracy	Â± 1 nm
Wavelength Bunching	2 - 400 nm
Slit Width	Fixed 4 nm slit
Diode Width	~ 0.5 nm

Setable Flow Range	Setpoints 0.001 to 5 ml/min, in 0.001 ml/min increments
Flow Range	0.1 to 5 ml/min
Flow Precision	< 0.07% RSD or < 0.02 min SD
Flow Accuracy	Â± 1% or 10 ul/min
Pressure	Operating range 0 to 400 bar (0 to 5880 psi) up to 5 ml/min
Pressure Pulsation	< 2% amplitude (typically < 1%) at 1 ml/min isopropanol, at all pressures > 1 MPa
Compressibility Compensation	User-selectable, based on mobile phase compressibility
Hydraulic System	Two dual pistons in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
Recommended pH Range	1.0 to 12.5; solvents with pH < 2.3 should not contain acides with attack stainless steel
Gradient Formation	High-pressure binary mixing, delay volume 180 to 480 ul without mixer, 600 to 900 ul with mixer, dependent upon back pressure
Composition Range	1 to 99% or 5 ul/min per channel
Composition Precision	< 0.5% absolute
Composition Accuracy	< 0.15% RSD at 1 ml/min

Maximum Flow Rate	0 to 5 ml/min per channel
Number of Channels	4
Internal Volume Per Channel	Typically 1ml per channel
Materials in Contact with Solvent	PTFE, FEP, PEEK
pH Range	1 to 14

Temperature Range	10Â°C below ambient to 80Â°C
Temperature Stability	Â± 0.15Â°C
Temperature Accuracy	Â± 0.8Â°C
Column Capacity	Three 30 cm columns
Warm-Up / Cool-Down Time	5 minutes from ambient to 40Â°C 10 minutes from 40Â°C to 20Â°C

Pressure	Up to 600 bar (8700 psi)
GLP Features	Early maintenance feedback (EMF), electronic records of maintenance and errors
Communications	Controller-area network (CAN). RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
Safety Features	Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
Injection Range	0.1 to 100 ul in 0.1 ul increments
Precision	Typically < 0.25% RSD of peak areas from 5 to 100 ul Typically < 1% RSD of peak areas from 1 to 5 ul
Carryover	Typically < 0.01 % using the following conditions: Column: 125 x 4 mm Hypersil ODS, 5 µm Mobile phase: Water/Acetonitrile = 80/20 Flow rate: 1 ml/min Injection volume: 1 ul caffeine (1 mg/ml), 5 ul water to test carryover Outside wash of needle before injection: 20 sec with water using flush port
Sample Viscosity Range	0.2 to 50 cp
Sample Capacity	2 × well plates (MTP) + 10 × 2 ml vials 108 x 2-mL vials in 2 x 54 vial plate plus 10 additional 2 mL vials 30 x 6-mL vials in 2 x 15 vial plate plus 10 additional 2 mL vials 54 Eppendorf tubes (0.5/1.5/2.0mL) in 2 x 27 Eppendorf tube plate
Injection Cycle Time	Typically < 30 s using following standard conditions: Default draw speed: 200 ul/min Default eject speed: 200 ul/min Injection volume: 5 ul

Pressure	Up to 1200 bar
GLP Features	Early maintenance feedback (EMF) for continuous tracking of instrument usage with user-settable limits and feedback messages. Electronic records of maintenance and errors
Communications	Controller-area network (CAN), RS-232C, APG Remote: ready, start, stop and shut-down signals, optional four external contact closures and BCD vial number output
Safety Features	Leak detection and safe leak handling, leak output signal for shutdown of pumping system, low voltages in maintenance areas, error detection and display
Injection Range	0.1 - 20 uL in 0.1 uL increments
Precision	Typically < 0.25% RSD of peak areas from 5 to 20 uL Typically < 0.5% RSD of peak areas from 2 to 5 uL
Carryover	Typically < 0.004%
Sample Viscosity Range	0.2 - 5 cp
Metering Devices	Metering pump in high pressure flow path
Sample Capacity	2 x well plates (MTP) + 10 x 2 mL vials 108 x 2 mL vials in 2 x 54 vial plate plus 10 additional 2 mL vials 30 x 6 mL vials in 2 x 15 vial plate 100 Micro vial tray, plus 10 additional 2 mL vials 54 Eppendorf tubes (0.5/1.5/2 mL) in 2 x 27 Eppendorf tube plate
Injection Cycle Time	Typically < 21 s with draw speed of 100 uL/min and ejection speed of 100 uL/min with injection volume of 5 uL

Pressure	0 – 60 MPa (0 – 600 bar, 0 – 8702 psi)
GLP Features	Early maintenance feedback (EMF) for continuous tracking of instrument usage with user-settable limits and feedback messages. Electronic records of maintenance and errors
Communications	Controller Area Network (CAN), Local Area Network (LAN) ERI: ready, start, stop and shut-down signals
Safety Features	Leak detection, safe leak handling, leak output signal for shutdown of pumping system, and low voltages in major maintenance areas
Injection Range	0.1 – 100 uL in 0.1 uL increments with 100 uL up to 60 MPa
Precision	< 0.25 % RSD of peak areas from 5 uL to 100 uL
Carryover	Typically < 0.004% with needle wash
Sample Viscosity Range	0.2 to 5 cP
Sample Capacity	132 x 2 ml vials 100 x 2 mL vials 36 x 6 mL vials
Injection Cycle Time	Typically 18 s for draw speed 200 uL/min and injections volume of 1 uL

Detection Type	Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis
Performance Specifications	Single Wavelength Operation: RAMAN (H2O) > 500 (noise reference measured at signal), Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell RAMAN (H2O) > 3000 (noise reference measured at dark value), Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell Dual Wavelength Operation: RAMAN (H2O) > 300 Ex 350 nm, Em 397 nm and Ex 350 nm, Em 450 nm, standard flow cell
Excitation Monochromator	Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 300 nm
Emission Monochromator	Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 400 nm
Wavelength characteristic	Repeatability: +/- 0.2 nm Accuracy: +/- 3 nm setting

Temperature Range	Ambient -10Â°C to 85Â°C (minimum 4Â°C)
Temperature Stability	Â± 0.1Â°C
Temperature Accuracy	Â± 0.5Â°C (with calibration for 40Â°C)
Column Capacity	4 columns of up to 300 mm length
Heat-Up / Cool-Down Time	5 min from Ambient to 40Â°C 10 min from 40Â°C to 20Â°C < 25 min from 25Â°C to 85Â°C

Settable Flow Range	0.001 to 5 ml/min, in 0.001 ml/min increments
Flow Precision	≤ 0.07 % RSD or 0.01 min SD, whichever is greater
Flow Accuracy	± 1 % or ± 10 uL/min, whichever is greater
Pressure Operating Range	Up to 80 MPa (800 bar, 11603 psi) up to 5 mL/min
Pressure Pulsation	< 1 % amplitude or < 0.5 MPa (5 bar), whichever is greater
Recommended pH Range	1.0 to 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
Gradient Formation	Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve
Delay Volume	≤ 350 µL (default configuration)
Composition Range	Settable range: 0 to 100 % Recommended range: 1 to 99 %
Composition Precision	< 0.15 % RSD or 0.02 min SD, whichever is greater
Integrated Degassing Unit	Number of channels: 4 Internal volume per channel: 1.5 ml

Temperature Range	Ambient -10°C to 100°C
Temperature Stability	± 0.05°C
Temperature Accuracy	± 0.8°C
Column Capacity	Three 30 cm columns
Warm-Up / Cool-Down Time	5 minutes from ambient to 40°C 10 minutes from 40°C to 20°C

Detection Type	Double-beam photometer
Light Source	Deuterium lamp
Wavelength Range	190 - 600 nm
Noise	< Â± 0.25 x 10^-5 AU, at 230 nm (single wavelength detection) < Â± 0.80 x 10^-5 AU, at 230 nm and 254 nm (dual wavelength detection)
Drift	< 1 x 10^-4 AU/h at 230 nm
Linearity	> 2.5 AU upper limit
Wavelength Accuracy	Â± 1 nm
Wavelength Precision	< Â± 0.1 nm
Slit Width	6.5 nm typical over whole wavelength range

Trigger Modes	Time slices, Peak (threshold, up- /downslope), Timetable (combination of time intervals and peak) and Manual trigger (supported only with G1323BControl Module)
Operating Modes	Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste
Test Tube/Plate Sizes	Minimum 48 mm to 75 mm maximum
Maximum Tube Volume	ca. 45 ml
Maximum Flow Rate	100 ml / min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve)
Delay Volumes [Î¼l]	Fraction collector inlet to diverter valve: -500 (typical, depends on length of the tubing) Diverter valve: ~15 Diverter valve to needle: ~110 Needle: -~5
Delay Calibration Sensor	Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode
Diverter Valve	3/2 Diverter valve with low internal volume (15 Î¼l), switching time < 100 ms, maximum operating pressure 6 bar
Maximum Capacity	3 fraction collectors in parallel plus one recovery fraction collector connected via 12-Position, 13-Port Selector valve
Interfaces	Controller-area network (CAN). optional; LAN or external contacts interface RS232C, APG-remote (for remote start / stop signals to / from other modules) Interface to G1330A Thermostat CAN-DC-out for operation of Agilent approved external devices like valves
Safety Features	Leak detection and safe leak handling, error detection and display, exhaust fan for fume extraction of hazardous vapors

Hydraulic System	Two dual piston in series pumps with servo-controlled variable stroke drive, power transmission by gears and ball screws, floating pistons
Setable Flow Range	0.001 to 5 mL/min, in 0.001 mL/min increments
Flow Range	0.05 to 5.0 mL/min
Flow Precision	< 0.07% RSD or < 0.02 min SD, whatever is greater
Flow Accuracy	Â± 1% or 10 Âµl/min, what ever is greater
Pressure Operating Range	0 to 60 MPa (0 to 600 bar, 0 to 8,700 psi) up to 5 mL/min
Pressure Pulsation	< 2% amplitude or < 3 bar at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar, 147 psi)
Compressibility Compensation	Pre-defined, based on mobile phase compressibility
Recommended pH Range	1.0 to 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
Gradient Formation	High-pressure binary mixing
Delay Volume	Â± 1% or 10 Âµl/min, what ever is greater Low delay volume configuration: 120 Âµl

GLP Features	Early maintainece feedback (EMF), electronic records of maintainence and errors
Communications	Controller-area network (CAN). RS232C, APG remote standard, optional four external contact closures and BCD vial number output
Safety Features	Leak detection and safe leak handling, low voltages in maintence areas, error detection and display
Injection Range	0.1 to 100 Âµl in 0.1 Âµl increments Up to 1500 Âµl with multiple draw (hardware modification required)
Precision	Typically < 0.25 % RSD from 5 â€š 100 Âµl, Typically < 1 % RSD from 1 - 5 Âµl
Pressure Range	400 bar (5800 psi)
Sample Viscosity Range	0.2 to 5 cp
Sample Capacity	2 Ã— well-plates (MTP) + 10 Ã— 2 ml vials 108 x 2 ml vials in 2 x 54 vial plate plus 10 additional 2 mL vials 30 x 6-mL vials in 2 x 15 vial plate plus 10 additional 2 mL vials 54 Eppendorf tubes (0.5/1.5/2.0mL) in 2 x 27 Eppendorf tube plate
Injection Cycle Time	Typically < 30 s using following standard conditions: Default draw speed: 200 Âµl/min Default eject speed: 200 Âµl/min Injection volume: 5 Âµl
Carry-Over	Typically < 0.01 % using the following conditions: Column: 125 x 4 mm Hypersil ODS, 5 Âµm Mobile phase: Water/Acetonitrile = 80/20 Flow rate: 1 ml/min Injection volume: 1 Âµl caffeine (1 mg/ml), 5 Âµl water to test carryover Outside wash of needle before injection: 20 sec with water using flush port

Hydraulic system	Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
Setable flow range	0.001 to 10 mL/min, in 0.001 mL/min increments
Flow range	0.05 to 10.0 mL/min
Flow precision	< 0.07% RSD or < 0.02 min SD, whatever is greater based on retention time at constant ambient temperature
Flow accuracy	Â± 1% or 10 Âµl/min whatever is greater
Pressure	Operating range 0 400 bar (0 to 5880 psi) up to 5 ml/min
Pressure pulsation	< 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa
Compressibility compensation	User-selectable, based on mobile phase compressibility
Recommended pH range	1.0 to 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
Gradient formation	High-pressure binary mixing, delay volume 180 â€š 480 Âµl without mixer, 600 to 900 Âµl with mixer, dependent on back pressure
Composition range	1 to 99 % or 5 Âµl/min per channel, whatever is greater
Composition precision	< 0.5% absolute
Composition accuracy	Â± 0.15% RSD, at 1 ml/min

Temperature Range	10Â°C below ambient to 100Â°C
Temperature Stability	Â± 0.05Â°C
Temperature Accuracy	Â± 0.8Â°C
Column Capacity	Three 30 cm columns
Warm-Up / Cool-Down Time	5 minutes from ambient to 40Â°C 10 minutes from 40Â°C to 20Â°C

Detection Type	Refractive Index
Refractive Index Range	1.00 to 1.75 RIU, calibrated
Measurement Range	Â± 600 x 10^-6 RIU
Short Term Noise	< Â± 1.25 x 10^-9 RIU
Drift	< 200 x 10^-9 RIU/hr
Valves	Automatic purge and automatic solvent recycle

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Agilent 1100 G1323B HPLC Gameboy Controller

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Settable Flow Range	Set points 0.001 to 10 ml/min, in 0.001 ml/min increments
Flow Range	0.2 to 10.0 ml/min
Flow Precision	â‰¤ 0.07% RSD, or â‰¤ 0.02 min SD whatever is greater, based on retention time at constant room temperature
Flow Accuracy	Â± 1% or 10 Âµl/min, whatever is greater, pumping degassed H₂O at 10 MPa (100 bar)
Pressure Operating Range	Operating range up to 60 MPa (600 bar, 8700 psi) up to 5 ml/min Operating range up to 20 MPa (200 bar, 2950 psi) up to 10 ml/min
Pressure Pulsation	< 2% amplitude (typically < 1.0%) or < 0.3 MPa (3 bar, 44 psi), whatever is greater, at 1 ml/min isopropanol, at all pressures > 1 MPa (10 bar, 145 psi)
Recommended pH Range	1.0 to 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
Gradient Formation	Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve
Delay Volume	600 to 900 Âµl, dependent on back pressure; measured with water at 1 ml/min
Composition Range	0 to 100% in 0.1% increments
Composition Precision	< 0.2% RSD or < 0.04 min SD, whatever is greater, at 1 ml/min; based on retention time at constant room temperature
Integrated Degasser	Number of channels: 4 Internal volume per channel: 1.5 ml
Dimensions (H x W x D)	7.1 x 15.6 x 17.2 in. (18 x 39.6 x 43.6 cm)
Electrical Requirements	100 to 240 V, 50/60 Hz, 80 VA

Injection Range	Default: 0.1 to 100 ÂµL in 0.1 ÂµL increments; optional: 20 ÂµL or 40 ÂµL (using 100 ÂµL analytical head)
Injection Precision	<0.2 % RSD or SD <10 nL, whatever is greater
Injection Linearity	0.9999 in the range of 0.1 to 100 ÂµL
Pressure Range	Up to 800 bar
Sample Viscosity Range	0.2 to 5.0 cP
Sample Capacity	1H Drawer: up to 8 drawers and 16 positions; Shallow well plates (MTP) 2H Drawer: up to 4 drawers and 8 positions; MTP, deep well plates, vials, Eppendorf 3H Drawer: up to 2 drawers and 4 positions; MTP, deep well plates, vials up to 6 mL, Eppendorf
Injection Cycle Time	<10 s using following standard conditions: Default draw speed: 100 ÂµL/min Default eject speed: 400 ÂµL/min Injection volume: 1 ÂµL
Carryover	<0.003 % (30 ppm) Multisampler Standard and Dual Needle <0.0009 % (9 ppm) Multisampler Multiwash
Multiwash	Outer needle wash and seat backflush for carryover reduction with up to 3 different solvents
Dimensions (H x W x D)	12.6 x 15.6 x 18.4 in. (32 x 39.6 x 46.8 cm)
Weight	48.5 lb. (22 kg.)
Electrical Requirements	100 to 240V, 50/60Hz, 180VA

Maximum Flow Rate	0 to 10 ml/min per channel
Number of Channels	4
Internal Volume Per Channel	0.45ml per channel
Materials in Contact with Solvent	TFE/PDD Copolymer, FEP, PEEK
pH Range	1 to 14

Pressure	Operating range 0 - 60 MPa (0 - 600 bar, 0 - 8700 psi)
GLP Features	Early maintenance feedback (EMF), electronic records of maintenance and errors
Communications	Controller-area network (CAN), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
Safety Features	Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
Injection Range	0.1 â€“ 100 ÂµL in 0.1 ÂµL increments (recommended 1 Âµl increments) Up to 40 ÂµL with reduced injection volume kit (hardware modification required) Up to 1500 Âµl with multiple draw (hardware modification required)
Precision	< 0.25 % from 5 â€“ 40 ÂµL < 0.5 % from 2 â€“ 5 ÂµL < 0.7 % from 1 â€“ 2 ÂµL < 1.5 % from 0.5 â€“ 1 ÂµL
Carryover	Typically < 0.004%
Sample Viscosity Range	0.2 â€“ 5 cp
Metering Devices	Metering pump in high pressure flow path
Sample Capacity	2 x well plates (MTP) + 10 x 2 ml vials 108 x 2 ml vials in 2 x 54 vial plate plus 10 additional 2 ml vials 30 x 6 ml vials in 2 x 15 vial plate 100 Micro vial tray 54 Eppendorf tubes (0.5/1.5/2 ml) in 2 x 27 Eppendorf tube plate
Injection Cycle Time	Typically < 21 s using default conditions and injections volume of 5 ÂµL

Pressure	Operating range 0 to 60 MPa (0 to 600 bar, 0 to 8850 psi)
GLP Features	Early maintenance feedback (EMF), electronic records of maintenance and errors
Communications	Controller-area network (CAN), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
Safety Features	Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
Injection Range	0.1 to 100 Âµl in 0.1 Âµl increments (recommended 1 Âµl increments) Up to 1500 Âµl with multiple draw (hardware modification required)
Replicate Injections	1 to 99 from one vial
Precision	Typically < 0.25 % RSD of peak areas from 5 to 100 Âµl Typically < 1 % RSD of peak areas from 1 to 5 Âµl
Minimum Sample Volume	1 Âµl from 5 Âµl sample in 100 Âµl microvial, or 1 Âµl from 10 Âµl sample in 300 Âµl microvial
Carryover	Typically < 0.1 %, < 0.05 % with external needle cleaning
Sample Viscosity Range	0.2 to 50 cp
Metering Devices	Metering pump in high pressure flow path
Sample Capacity	100 x 2 ml vials in 1 tray 40 x 2 ml vials in Â½ tray 15 x 6 ml vials in Â½ tray (Agilent vials only)
Injection Cycle Time	50 seconds for draw speed 200 Âµl/min, ejection speed 200 Âµl/min, injection volume 5 Âµl

Delay Volume (in ul)	Fraction collector inlet to diverter valve: ~50 (typical, depends on length of the tubing) Diverter valve: ~15 Diverter valve to needle: ~10 Needle: ~4
Maximum System Flow	10 mL/min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve). The analytical scale fraction collector can be modified for flow rates > 10 ml/min
Maximum Collection Volume	~20 mL with 48 mm tubes ~30 mL with 75 mm tubes
Maximum Capacity	3 fraction collectors in parallel plus one recovery fraction collector connected via 12-Position, 13-Port Selector valve (PN G1160A)
Cooling	Optional (with additional G1330B), performance depending on ambient conditions and the volume of collected fractions
Trigger Modes	Time slices Peak (threshold, up- / downslope) Timetable (combination of time intervals and peak) Manual trigger (supported only with Agilent Instant Pilot G4208A)
Trigger Sources	Agilent 1260 Infinity DAD/MWD detectors (G1315C,D; G4212B/ G1365 C,D), the Agilent 1260 Infinity Fluorescence Detector (G1321A) and the Agilent G1946C/D, G1956A/B LC-MSD are fully supported
Operating Modes	Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using deep well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste Needle into location: Needle pushes into the vessel as deep as specified, for the use with capped vials and test tubes and well plates with closing mats Droplet setup mode: The tip of the fraction collector needle will initially move down to the bottom of the well. Then it will slowly move upwards while the fraction is collected. The droplet setup mode enables the fraction collector to collect small fractions without bubbles
Diverter Valve	3/2 valve, with switching time < 100 ms
Maximum Pressure	6 bar at the diverter valve during switching
Plates/Trays	4 x well-plates full tray (MTP)* 2 x well-plates std. tray + 10 funnels with external containers* (+ 1 half tray) 2 Ã— well-plates std. tray (MTP) + 10 Ã— 2 ml vials* (+ 1 half tray) 100 x 2 ml in std. tray (+ 1 half tray)* 3 x 40 x 2 ml in half tray* 3 x 40 funnels in half tray 3 x 15 x 6 ml in half tray* Full tray with 40 test tubes (30 mm OD, max. height 48 mm, ~20 ml vol.) Full tray with 60 test tubes (25 mm OD, max. height 48 mm Full tray with 126 test tubes (16 mm OD, max. height 48 mm) Full tray with 215 test tubes (12 mm OD, max. height 48 mm)
Maximum Tube Height	48 mm with long needle assembly G1367-87200 75 mm with short needle assembly G1364-87202
Delay Calibration Sensor	Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode
Diverter Valve	3/2 Diverter Valve with low internal volume (15 ul), switching time < 100 ms, maximum operating pressure 6 bar